Molecular Mechanisms for Repair of DNA: Part A by Peter A. Cerutti (auth.), Philip C. Hanawalt, Richard B.

By Peter A. Cerutti (auth.), Philip C. Hanawalt, Richard B. Setlow (eds.)

An "age" has handed within the forty years due to the fact we first saw restoration from radiation harm in irradiated micro organism. throughout the early Nineteen Thirties, we have been discussing the opportunity of speedy alterations after radiation publicity with Farring­ ton Daniels, Benjamin Duggar, John Curtis, and others on the collage of Wisconsin. After operating with residing cells, we had concluded that organisms receiving substantial insults should have a large choice of fix mechanisms to be had for recovery of no less than a number of the crucial houses of the telephone. the matter used to be the way to fmd and determine those restoration phenomena. at the moment i used to be engaged on an issue thought of to be of significant importance-the life of the so-called mitogenetic rays. numerous hundred articles and a ranking of books had already seemed facing mitogenetic rays, a kind of radiation that was once proposal to exist within the shorter ultraviolet sector. Our look for mitogenetic rays necessitated the layout of experiments of maximum sensitivity for the detection of ultraviolet. It used to be important that stipulations be saved as consistent as attainable in the course of publicity. all of the paintings was once performed at icewater temperature (3-5°C) in the course of and after publicity. We knew that gentle was once an enormous issue for cellphone restoration, so all our experiments have been performed in dim gentle, with the plated-out cells being coated with darkish fabric. Our statements at the impact of seen mild encouraged Kelner to go looking for "photoreactivation' (as it was once later called).

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22% residual thymidine incorporation. Protocol: 0 min, add HU; 30 min, add AAAF; 90 min, add [3H I dThd; 150 min, harvest. 23 Nature of Alkylation Lesion Table 4. 5 mM MMS for 1 hr. 1 mM [ 14 C 1MMS for 2 hrs. 5 to make the values comparable. 2 cells The amount of methylation was determined relative to the amount of DNA determined by uniform labeling with I' H 1dThd at a specific activity of 3 Ci/mmole. The [ 14 C 1MMS had a specific activity of 46 mCi/mmoie. 2 for 3H. If the number of nucleotides inserted per lesion by excision-repair is very small, then bromodeoxyuridine may be a bad tracer for the study of alkylationinduced damage.

3 X 10-3 of the apurinic sites to be hydrolyzed. 9 X 10 1 single-strand breaks per hour. 8 X 10 8 nuc1eotides plus one cross·link per 7 X 10 7 nucleotides. 3 per replicon per hour. , 1974; Hadi and Goldthwait, 1971), it might seem reasonable to consider the apurinic site as the effective toxic alkylation lesion in cells. The assumption that spontaneously produced apurinic sites are the significant lesions in alkylated cells requires that new damage be continually produced. However, the data of Scudiero and Strauss (1974), Coyle et al.

J. Radiat. Bioi. 35,413--417. Roti Roti, J. and Stein, G. and Cerutti, P. (1974). Biochemistry 13, 1900--1904. Setlow, R. and Carrier, W. (1973) Nature New Bioi. 241,170--172. Setlow, R. and Setlow, J. (1972). Ann. Rev. Biophys. Bioeng. 1,293-346. , Lazurkin, Y. and Frank-Kamenetskii, M. (1973). Nature New BioI. 241,58--60. 12 Peter A. Cerutti Smets, L. and Comelis, T. (1971). Int. J. Radiat. BioI. 19,445-457. Smith, K. (1975). In Photochemistry and Photobiology of Nucleic Acids (Wang, S. Y. ).

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