By Dieter Marmé (auth.), A. J. Trewavas (eds.)
This quantity summarises the lecture and poster periods of a NATO complicated workshop held in Edinburgh, July 15th-19th, 1985. The workshop was once held to compile plant scientists of many alternative disciplines yet who percentage a typical curiosity within the regulatory position of calcium in plant improvement. even if this quantity covers the formal lawsuits, an equivalent size of time was once dedicated to dialogue either in huge and small teams. a bit of the flavor of the instructions and personality of the discussions may be present in the ultimate article by way of David Clarkson which was once written to hide this different clever exposed zone of the workshop. the amount displays a lot of the present pleasure within the box of plant calcium study. the various members are pioneers of their res pective parts and the level to which the final 5 years has visible a drama tic unfolding, a whole inversion of the function of calcium from basic macro nutrient to significant metabolic and developmental controller is stated the following. the fabric is new and lots more and plenty of it unpublished. In plant body structure, the eighties could but be special the last decade of calcium.
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Extra info for Molecular and Cellular Aspects of Calcium in Plant Development
In this paper we extend our observations to other species, we report on the presence of significant levels of calmodulin in sieve tube sap and plant extracts, and we present data on calcium levels in the sieve tube. MATERIAL AND METHODS Plants: The species used for analysis of calcium-binding or calmodulin levels were Cucurbita maxima Duch. cvs. Golden Delicious and Gelber Zentner, Cucurbita pepo L. cv. Long Green Trailing, Cucumis sativus L. cv. Telegraph, Cucumis melo L. cv. Blenheim Orange and Zea mays L.
Cv. Long Green Trailing, Cucumis sativus L. cv. Telegraph, Cucumis melo L. cv. Blenheim Orange and Zea mays L. cv. Golden Bantam. Exudate: Phloem exudate from cucurbit species was collected either by slicing and rinsing the stem and immersing the cut end into collection buffer, or by using microcapillaries and a graduated microsyringe and expelling the exudate into buffer. 14 M 2-mercaptoethanol or 25 mM iodoacetamide. 5. The exudate samples were usually centrifuged at 20,000 g for 20 min at 4°C,the pellets discarded and the supernatant fractions desalted by centrifugation through Sephadex 8-25.
J. Lukas, M. Wiggins and D. M. Watterson, Amino Acid Sequence of a Novel Calmodulin from the Unicellular Alga Chlamydomonas. Plant Physiol 78:477-483 (1985). M. Schleicher, T. J. Lukas and D. M. Watterson, Further Characterization of Calmodulin from the Monocotyledon Barley (Hordeum vulgare). Plant Physiol 73, 666-670 (1983). D. R. Marshak, M. Clarke, D. M. Roberts and D. M. Watterson, Structural and Functional Properties of Calmodulin from the Eukaryotic Microorganism Dictyostelium discoideum.