By Eeva Therman;Millard Susman
This ebook, just like the earlier versions, was once written as an creation to human cytogenetics, however it may be used as a textual content for a basic cytogenetics path, due to the fact that chromosome constitution and behaviour are related in all eukaryotes. Many examples during this booklet are from organisms except people, reflecting our mixed backgrounds of molecular and bacterial genetics, and plant and animal cytogenetics. within the speedily increasing box of human cytogenetics, yes matters, for example scientific and melanoma cytogenetics, are actually lined in lately released, thousand-page volumes. during this ebook, such topics are offered purely in define. the big development of data has additionally made the alternative of subject matters and of examples to demonstrate them much more arbitrary and subjective than within the prior versions. except a number of pages right here and there, the textual content has been rewritten. significant elements, in particular these on molecular concerns, were extra. This e-book wouldn't exist with out the devoted participation of Mrs. Barbara Susman. She has been all in favour of the undertaking from the making plans levels to the ultimate proofreading. She has performed the vast literature study, designed lots of the tables and illustrations, and edited and typed the textual content. For discussions and proposals we're indebted to many colleagues. we want specially to say Drs. Lassi Alvesalo, Evelyn M. Kuhn, and Renata Laxova, who've seriously learn chosen components of the ebook, and Dr. Carter Denniston, who has learn the total text.
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Extra resources for Human Chromosomes: Structure, Behavior, and Effects
T . ' . "~'. r'.... " ... ~ .. _. .. '1i. _... 1,- 'C \'~ . :.. it .... ". :;~ . ' . ''¥'. ,:..... ,.. .. ~ . \. : ~c-'! ~ .... 3, Semiconservative replication of human chromosomes corresponding to Fig. 2 (see text). radioactive constituent of DNA, usually [3H]thymidine, and are then transferred back to normal nonradioactive medium. The results of such an experiment can be seen in Figs. 2 and 3 (Taylor, 1963). Before the S period, the chromosome consists of one double helix of DNA. During the S period, in radioactive medium, each strand acts as a template for a new radioactive strand.
Annu Rev Biophys Bioeng 5:1-37 Martin RH (1989) Invited editorial: segregation analysis of trans locations by the study of human sperm chromosome complements. Am J Hum Genet 44:461463 Martin RH, Rademaker A W, Hildebrand K, et al. (1987) Variation in the frequency and type of sperm chromosomal abnormalities among normal men. Hum Genet 77:108-114 Mendelsohn ML (1966) Absorption cytophotometry. Comparative methodology for heterogeneous objects and the two-wavelength method. In: Wied GL (ed) Introduction to quantitative cytochemistry.
Slides stained with quinacrine are not permanent, and after a couple of photomicrographic exposures the fluorescence fades too much to be usable. The other type of banding technique involves various pretreatments and staining with Giemsa or certain fluorochromes; Giemsa bands (G- Banding Techniques 37 bands or GTG bands) are obtained when the chromosomes are pretreated with a salt solution at 60°C or with proteolytic enzymes, usually trypsin (Drets and Shaw, 1971). Giemsa banding yields essentially the same information as Q-banding, only the brightly fluorescent Q-bands are now darkly stained, whereas the Q-dark regions are light (Fig.