Gene Regulation by Steroid Hormones by Gerald C. Mueller (auth.), Arun K. Roy, James H. Clark

By Gerald C. Mueller (auth.), Arun K. Roy, James H. Clark (eds.)

Within the final 20 years endocrinological learn has taken a distinct tum towards biochemistry and molecular biology. This has led to a brand new self-discipline referred to as' 'molecular endocrinology. " experiences at the mechanism of hormone motion have persevered to make headlines with basic discov­ eries in receptor motion and gene legislation. lately the insect endocrino­ logists have additionally started to discover the molecular mechanism of steroid hor­ mone motion profiting from the giant variety of Drosophila mutants, the library of Drosophila gene, and several other well-characterized cell-lines. the provision ofthe recombinant DNA know-how has supplied a very revolu­ tionary instrument within the fingers of the molecular endocrinologists. "Gene Regula­ tion by means of Steroid Hormones" is compiled and provided during this frontier spirit, and we are hoping that this quantity will serve not just the lively investigators within the box yet can also be very necessary to scholars and researchers with a gen­ eral curiosity in regulatory biology. The booklet is an offshoot of the convention on Molecular Mechanism of Steroid Hormone motion held on the Meadow Brook Mansion of Oakland college within the fall of 1978. we want to recognize the monetary help­ ance from the nationwide technology starting place and Oakland collage. The conferees won't ever overlook the warmest hospitality of Dr. LOWELL EKLUND and his employees on the Meadow Brook middle and we additionally desire to exhibit according to­ sonal gratitude to lots of our scholars and associates for aiding us to make the convention a very good success.

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A, B, c o, , E, F, -origin Fig. 9. Identification of ovomucoid mRNA sequences in high molecular weight nu- clear RNA. A oviduct nuclear RNA, 20 J-Lg (high molecular weight ovomucoid RNA species "c" and "f' were not detected in this preparation). B Ovomucoid mRNA purified to approximately 40%, 15 ng. C Oviduct cytoplasmic RNA, 20 J-Lg. D Spleen nuclear RNA, 20 J-Lg. E liver nuclear RNA, 20 J-Lg. F RNA isolated after mixing liver nuclei with oviduct cytoplasm, 20 J-Lg. The position of 28S, 23S, I8S, and 16S ribosomal RNA markers is indicated .

The experiments were carried out and the data analyzed as described in the legend for Fig. 7. The data demonstrate that after primary (0) or secondary (e) stimulation, the mRNA accumulated for a period of 3 days. Its rate of disappearance during the next 7 days was consistent with that expected for a molecule with a half-life of approximately 30 h. 5 times higher than that reached following primary stimulation. ) IV. The Natural Vitellogenin Gene Since we are ultimately interested in studying regulation of the vitellogenin gene in vitro, we were anxious to find out something about the structure and organization of the vitellogenin gene in the chicken genome, and to clone DNA sequences that would provide potential substrates and probes for in vitro transcription studies.

Overall scheme employed in cloning double-stranded cDN A. ) complementary tails, and the circularized molecules are used to transform the enfeebled E. coli host, X1776 (Bolivar et al. 1977). Note that following transformation the host repairs the two PstI sites, allowing the subsequent excision of the DNA with this enzyme. We now have a collection of vitellogenin cDNA clones and are using them in studies that are still too preliminary to discuss here. 45 Effect of Estrogen on Gene Expression in Avian Liver VI.

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