Cell Impairment in Aging and Development by L. M. Franks (auth.), Vincent J. Cristofalo, Emma Holečková

By L. M. Franks (auth.), Vincent J. Cristofalo, Emma Holečková (eds.)

lar getting older, to which this version contributes, has grown. except experiences on paintings during this virtually "classical" diploid phone method, the symposium offers reports utilizing varied organic structures with effects which were profitable as info is received on styles of swap which are universal to a couple of experimental approach. certainly, lately even more has been realized concerning the destiny of all kinds of intermitotic and postmitotic cells in situ. The symposium has additionally offered contributions dealing, in some way with getting older yet with early ontogeny; such details on early developmental alterations may still definitely make clear a few of the mechanisms serious about getting older. we're cognizant of the truth that environmental impacts due to the complexities of contemporary civilization could have effects that purely happen a lot later, and profoundly have an effect on the lifespan of the organism. There stay, after all, many unanswered questions. no matter if there's "physiological" instead of "pathological" getting older; even if "old" cultures dwelling in unchanged, even though now not exhausted, medium, are degenerating, no longer getting older; what's concerned whilst "old" fragment cultures regenerate after excision via filling the wound with "young" cells; why a few tumor cells in vivo in addition to in vitro die whereas others stay; all are questions~eserving of our attention.

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These data led to the kinetic model for human fibroblasts, illustrated schematically in Figure 1. Early possoqe Days otter SlIbcuItivation Intermediate pOSSQ911 Days after subcultivation so1urotlon ~si ty Late poswoe :') ) ))))) Days after subcu/tivolion Fig. 1. Cell kinetics of human embryonic fibroblasts at 3 different passage levels. Each circle or ellipse represents the generation time of a cell. 'ID METABOLIC CHANGES 53 saturation density. On the right hand side are represented the growth curves that are obtained at the corresponding passage levels.

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The initiation of cell division in a contact-inhibited mammalian cell line. Compo Physiol. 66: 325. J. CRISTOFALO 12. W. J. 1967. Androgen inhibition of mammary gland differentiation in vitro. Endocrinol. 80: 329. 13. A. and Piddington, R. 1966. Stimulation by hydrocortisone of premature changes in the developmental pattern of glutamine synthetase in embryonic retina. Biochim. Biophys. Acta 121: 409. 14. Reif-Lehrer, L. and Chader, G. 1972. Hormonal effects on the neural retina: corticoid uptake, specific binding and structural requirements for the induction of glutamine synthetase.

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