Bacteria from fish and other aquatic animals: a practical by Nicky B. Buller

By Nicky B. Buller

This guide is split into sections essentially in keeping with the stairs taken to isolate and establish an unknown bacterium. skilled microbiologists may possibly locate a few sections uncomplicated, yet those are integrated for the advantage of scholars and newly graduated microbiologists or for researchers who're strange with the recommendations of bacteriology, rather these utilized in a diagnostic laboratory.

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Yellow, pus-filled fluid in pleural cavity 6. Recovered from healthy animals 1. Lymph nodes 2. Cutaneous lesions 3. Intestine, kidney, lung, spleen 4, 5. Isolated from various tissues 6. Lung 7. Liver, lung, brain and spleen of stranded porpoise 9. Uterus Tissue site 1. Brucellosis 2, 3. Associated with respiratory disease 4. Pneumonia – opportunistic infection in seal morbillivirus infection 5, 6, 7,8. Pathogenicity not known 1. Melioidosis 2. Clostridial myositis 3. Bacterial pneumonia, respiratory infection Pathogenicity not conclusive 4.

90. Vibrio mimicus, Gram stain. Fig. 95. Vibrio parahaemolyticus on BA, 24 h, 25oC. Fig. 91. Vibrio ordalii on MSA-B, 2 days. Fig. 96. Vibrio parahaemolyticus on TCBS, 24 h. Fig. 92. Vibrio ordalii on BA, 3 days, 25oC. No growth on TCBS. Fig. 97. Vibrio parahaemolyticus. Gram smear. Fig. 93. Vibrio ordalii, Gram stain. Fig. 101. Vibrio tubiashii on MSA-B, 2 days, 25oC. Fig. 98. Vibrio proteolyticus on BA, 24 h, 25oC. Showing swarming colonies. Fig. 102. Vibrio tubiashii on TCBS, 3 days, 25oC. Fig.

107. Yersinia ruckeri on BA, 24 h, 25oC. Fig. 109. Vibrio identification discs. Top = 150 µg disc (sensitive). Bottom = 10 µg disc (resistant). Fig. 111. Carbohydrate fermentation reaction. Sucrose-positive (yellow) and sucrose-negative (red), 24 h, 25oC. Fig. 115. DNase reaction. Negative reaction on left and positive reaction on right. Fig. 112. Citrate test. Yersinia ruckeri, citrate-positive (blue) at 24oC, but citrate-negative (green) at 37oC. Fig. 118. Methyl Red reaction. Positive. Fig. 114.

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